Node Status
Learning Objectives
Describe the laboratory method of culturing TB bacteria and its historic utility in diagnosing TB and that it still continues to be the gold standard diagnostic test for TB.
Discuss the solid and liquid culture types along with its major advantages/ disadvantages.
Discuss that Culture laboratories are setup in only advanced laboratories in select locations in the country.
Sub Nodes
- Culturing of Mycobacteria: Process of Preparation of 0.067M Phosphate Buffer
- Culturing of Mycobacteria: Labelling of Reagents
- Precautions for Sample Processing and Reagent Preparation in TB Culture Labs
- MGIT 960 System as an Instrument for Culture
- MGIT 960 Instrument: Principle for Culture
- MGIT 960 Instrument: Parts
- MGIT 960 Instrument: Maintenance
- Culture Specimen Processing: Advantages and Disadvantages of NALC-NaOH Method
- Procedure for Culture Specimen Processing: Pulmonary specimens
- Procedure for Culture Specimen Processing: Extrapulmonary specimens
- Procedure for Culture Specimen Processing: Pus and other muco-purulent specimens
- Procedure for Culture Specimen Processing: Gastric Aspirate
- Inoculation of Brain Heart Infusion [BHI] Agar
- MGIT 960 Instrument: Operation
- Quality Control of MGIT 960 Tubes
- Steps to be done after Culture Specimen Processing
- BACTEC MGIT 960 Reagents and Storage
- BACTEC MGIT 960 Tube with Media [7 ml]
- BACTEC MGIT 960 Growth Supplement
- BACTEC MGIT 960 PANTA
- Specimen Processing for TB Cultures
- Procedure for Culture Specimen Processing: Bronchial Lavage
- Procedure for Culture Specimen Processing: Laryngeal Swab
- Procedure for Culture Specimen Processing: Tissue
- Procedure for Culture Specimen Processing: Urine
- Procedure for Culture Specimen Processing: CSF and other body fluids
- Preparation of PANTA for MGIT TB Cultures
- Preparation of MGIT Tubes for MGIT TB Cultures
- Inoculation of MGIT Tubes
- Incubation of MGIT Tubes
- Precautions for Inoculation and Incubation of MGIT Tubes
- Identifying Breaks in Liquid Culture Protocol
- BACTEC MGIT 960 System Growth Detection
- Work-up for Positive MGIT Cultures
- Interpreting MGIT Results
- Liquid Culture Contamination and sources of contamination
- Monitoring Liquid Culture Contamination
- False Positive MGIT Cultures
- False Negative MGIT Cultures
- Impact of False MGIT Culture Results
- Troubleshooting LC Growth Recovery
- Troubleshooting LC Detection Time
- Troubleshooting High Liquid Culture contamination
- Decontamination of Contaminated MGIT Culture
- Documentation of MGIT Cultures
- MGIT Culture Reading Schedule
- MGIT 960 Culture Detection Time Frame
- MGIT 960 Instrument Reports
- Reading MGIT 960 Tubes Manually
- LC Identification Methods for MTB/Isolated Mycobacteria
- MTB growth characteristics and morphology
- LC AFB Smears
- Rapid Antigen Detection/Immunochromatographic Assay for MTB
- Procedure for Immunochromatographic Test SD Bioline Assay/Capillia/TBcID
- Inoculum Preparation for Immunochromatographic Test SD Bioline Assay/Capillia/TBcID
- Results interpretation for Immunochromatographic Test SD Bioline Assay/Capillia/TBcID
- Internal Quality Control Kits for Immunochromatographic Test SD Bioline Assay/Capillia/TBcID
- Biochemical Tests for MTB
- Susceptibility of MTB to p-Nitrobenzoic acid (PNB)
- Niacin production test for MTB
- Nitrate reduction test for MTB
- Catalase peroxidase test for MTB
- Storage of Culture Isolates
- Media and consumables required to store culture isolates
- Preparation of Selective Middlebrook- 7H9 Liquid Medium
- Quality Control of Selective Middlebrook- 7H9 Liquid Medium
- Inoculation Procedure for Storage of Culture Isolates
- Sterility Check of Culture Isolates
- Incubation and Storage of Culture Isolates
- Revival of the Frozen Cultures
- MTB Drug Resistance
- Mechanism of action-anti TB drugs first line and second line
- Drug Susceptibility Methods
- Requirements for submission, acceptance and processing for culture Isolates
- Acceptance of MTB Isolates for DST
- Preparing the Isolate for DST
- Preparing the Isolate for Liquid Culture DST
- SIRE Susceptibility Testing
- Critical Concentrations and Reconstitution of SIRE Drugs
- Pyrazinamide PZA Susceptibility Testing
- Critical Concentration and Reconstitution of PZA
- Second-line Phenotypic Drug Susceptibility Testing
- Critical Concentration and formulations of Second-line Anti-TB Drugs for DST
- Preparation and Storage of Second-line Anti-TB Drugs used for DST
- Inoculum Preparation for First and Second-line Phenotypic DST
- Inoculum Preparation from MGIT Positive Culture
- Inoculation of Growth Control Tube/Drug Containing Tubes
- PZA Inoculum Preparation
- Inoculation of PZA Growth Control/Drug Containing Tube
- Incubation of MGIT 960 DST Sets
- Principle of MGIT DST Reading
- Procedure for Reading MGIT DST Results
- Unloaded MGIT DST Report
- Validation of Resistant/Unexpected MGIT DST Results
- LC DST Recording and Reporting
- Paper-based Format: NTEP Laboratory Register for CBNAAT and C&DST
- Drug Susceptibility Testing Logs
- Final Result Reporting
- MGIT 960 DST Equipment and Reagents Troubleshooting
- MGIT 960 DST Inoculum Troubleshooting
- Troublsehooting Invalid DST Sets " X"
- Troubleshooting Non-interpretable DST Results
- Troubleshooting False Susceptible DST Results
- Troubleshooting False Resistant DST Results
- Troubleshooting Unexpected/False Profile of DST Isolates
- Retesting MGIT DST Results
- MGIT DST Quality Control
- Standard Controls
- Procedure of Quality Control
- Quality Control of the MGIT DST Inoculum
- Quality Control in DST results interpretation
- Quality Assurance in TB C&DST Laboratories
- C&DST Laboratory Arrangement and Administration
- Pre-examination QA Procedures in C&DST Laboratories
- Examination QA Procedures in C&DST Laboratories
- Post-examination QA Procedures in C&DST Laboratories
- Quality Improvement Activities in C&DST Laboratories
- External Quality Assurance for C&DST laboratories
Comments
Potential duplicate with batch pages
Namrata Thu, 10/03/2022 - 14:03
Potential duplicate with batch pages:
Objective seems to match with Approved page 30767
Namrata Thu, 10/03/2022 - 14:05
In reply to Potential duplicate with batch pages by Namrata
Objective seems to match with Approved page 30767 Please update LMS link