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The Truenat is a rapid molecular diagnostic method based on the Nucleic Acid Amplification Testing (NAAT) principle. Truenat uses portable, battery-operated devices to rapidly detect Mycobacterium tuberculosis Complex Bacteria (MTBC) and Rifampicin resistance.
Truenat system involves two main devices:
- Trueprep® AUTO v2 Universal Cartridge based Sample Prep Device: Used for the automated extraction and purification of DeoxyriboNucleic Acid (DNA).
- Truelab® Real Time micro-PCR Analyzer: Used for performing Real-Time Polymerase Chain Reaction (RT-PCR), resulting in the semi-quantitative detection of MTBC.
The errors in Truenat are broadly categorised as:
- Errors with codes (Trueprep and Truelab errors)
- Errors without codes (System failures)
The errors without codes in Truenat and the ways to resolve them are listed in the table below:
| Sl. No. | System failures | Reason | Troubleshoot |
| 1 | ‘Unable to read chip information’ | Analyzer was unable to read chip memory | Check if the chip was loaded properly into the tray. Remove the chip and re-select the profile from Status Screen and repeat the steps. If the message reappears, load a new chip and re-load the elute again |
| 2 | ‘Could not initialize. Please try again’ | The system was unable to establish an internal connection | Attempt the test again by using a new chip and re-loading the elute again |
| 3 | “Chip is already used” OR “Chip loaded is expired” | User loaded a used chip/expired chip in the tray | Use a fresh chip and re-load the elute |
| 4 | Login attempt failure | Login failed due to incorrect password entered | Re-enter the correct password |
| 5 | Invalid patient name in sample details screen | The patient’s name in the sample details section is blank | Fill patient’s name in the sample details section |
Resource
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Guidelines on Programmatic Management of Drug-resistant TB (PMDT) in India; CTD, MoHFW, India. 2021.
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Trueprep AUTO v2 Universal Cartridge Based Sample Prep Device; User Manual V4.
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TRUELAB UNO Dx Real Time Quantitative micro PCR Analyzer; manual VER 04.
Assessment
|
Question |
Answer 1 |
Answer 2 |
Answer 3 |
Answer 4 |
Correct answer |
Correct explanation |
Page id |
Part of Pre-test |
Part of Post-test |
|
What should you do when you get a system failure alert as: “ Chip is already used” ? |
Re-insert the same chip |
Insert a fresh chip |
Do not insert any chip |
None of the above |
2 |
When you get a system failure alert as: “ Chip is already used” , Use a fresh chip and re-load the elute. |
|
Yes |
Yes |
|
What should you do when you get a system failure alert as: "Unable to read chip information" |
Re-insert the same chip |
Insert a fresh chip |
Do not insert any chip |
Check if chip is loaded properly, remove the chip and reselect the profile from status screen and repeat the steps. |
4 | When you get a system failure alert as "Unable to read chip information" Check if the chip was loaded properly into the tray. Remove the chip and re-select the profile from Status Screen and repeat the steps. If the message reappears, load a new chip and re-load the elute again |
Yes |
Yes |
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