Interpretation of FL-LPA: Predicting TB Drug-resistance

Zone developed/ not developed (failing) in Wild Type (WT) and Mutation (MUT) probes are used to predict drugs resistance in First Line - Line Probe Assay (FL-LPA).

For Rifampicin

Table 1 shows mutations in rpoB gene and corresponding WT, MUT and responsible codons and mutations.

• rpoB WT1-8: rpoB wild type probes
• rpoB MUT1-3: rpoB mutation probes

Table 1: Mutations in the rpoB gene and corresponding wild type and mutation band; Source: GenoType MTBDR plus ver 2.0 kit, Instructions for Use.

For Isoniazid

Table 2 and Table 3 show mutations in katG gene and inhA promoter region, respectively and the corresponding WT and MUT bands.

• katG WT: katG wild type probe
• katG MUT 1-2: katG mutation probe
• inhA WT 1-2: inhA wild type probe
• inhA MUT 1-2, MUT 3A, MUT 3B: inhA mutation probe

Table 2: Mutations in katG gene and corresponding WT, MUT bands and responsible codons and mutations; Source: GenoType MTBDR plus ver 2.0 kit, Instructions for Use.

Table 3: Mutations in inhA promoter region and corresponding WT, MUT bands and responsible codons and mutations; Source: GenoType MTBDR plus ver 2.0 kit, Instructions for Use.

Resources

• GenoType MTBDR plus ver 2.0 kit, Instructions for Use.
• GLI Line Probe Assays for Drug-resistant Tuberculosis Detection.

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